Journal: Journal of Translational Medicine

Article Title: RUNX1 knockdown induced apoptosis and impaired EMT in high-grade serous ovarian cancer cells

doi: 10.1186/s12967-023-04762-8

Figure Lengend Snippet: The expression comparison of RUNX1 in ovarian cancer. The expression pattern of RUNX1 in ovarian tumor tissues and NT (normal tissues) ( A ), including 426 tumor tissues (TCGA) and 88 normal tissues (GTEx). The comparison of the expression level for RUNX1 mRNA was performed. OS ( p = 0.0062, B ) of ovarian cancer patients was significantly positively associated with the expression of RUNX1. Representative images are shown the expression of RUNX1 in normal ovarian tissues, mucinous ovarian cancer tissues, LGSOC (low grade serous ovarian cancer) tissues, HGSOC (high grade serous ovarian cancer) tissues and other types of ovarian cancer tissues ( C ). The IHC staining scores were statistically analyzed between normal and ovarian cancer tissues ( D ). The overexpression of RUNX1 was analyzed by western blot in normal ovarian epithelial cells and HGSOC cell lines ( E and F )

Article Snippet: Sections were incubated overnight with RUNX1 antibody (Cusabio, China) at 4 °C.

Techniques: Expressing, Comparison, Immunohistochemistry, Over Expression, Western Blot

Journal: Journal of Translational Medicine

Article Title: RUNX1 knockdown induced apoptosis and impaired EMT in high-grade serous ovarian cancer cells

doi: 10.1186/s12967-023-04762-8

Figure Lengend Snippet: The RUNX1 mediated the abnormal proliferation, invasion, and migration in the ovarian cancer cells. Western blot confirmed RUNX1 stable knockdown in SKOV3 cells constructed by plasmids containing RUNX1-targeting shRNA ( A ). CCK8 assay showed that knockdown of the expression levels of RUNX1 reduced the proliferation ability of SKOV3 ( B ). Western blot confirmed RUNX1 stable knockdown in OVCAR3 cells constructed by plasmids containing RUNX1-targeting shRNA ( C ). CCK8 assay showed that knockdown of the expression levels of RUNX1 reduced the proliferation ability of OVCAR3 ( D ). Differences in the migration and invasion ability of cells after the knockdown of RUNX1 expression levels and the migration and invasion ability of the cells were decreased when RUNX1 was knocked down ( E and F , 200 ×). Colony formation assay showed that knockdown of the expression levels of RUNX1 reduced the ability of colony formation of SKOV3 and OVCAR3 ( G and H ). A representative experimental result was generated from three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to control cells expressing a scramble shRNA control, paired t-test

Article Snippet: Sections were incubated overnight with RUNX1 antibody (Cusabio, China) at 4 °C.

Techniques: Migration, Western Blot, Knockdown, Construct, shRNA, CCK-8 Assay, Expressing, Colony Assay, Generated, Control

Journal: Journal of Translational Medicine

Article Title: RUNX1 knockdown induced apoptosis and impaired EMT in high-grade serous ovarian cancer cells

doi: 10.1186/s12967-023-04762-8

Figure Lengend Snippet: Functional and pathway enrichment analysis of the RUNX1 knockdown expression profiling in ovarian cancer cells. Heat map demonstrated the significantly changed genes hierarchical cluster analysis of RUNX1 knockdown transcription profiles in SKOV3 ovarian cancer cells. The significantly changed genes of RUNX1 vs control were screened and identified ( A ). Representative Molecular Complex Detection (MCODE) network node demonstrated the connection of significantly changed genes regulated by RUNX1 knockdown ( B ). Representative Molecular clusters were enriched. Left panel, heatmap of the top 20 enriched terms ( C ). Metascape analysis revealed a Network of enriched sets colored by ID. Threshold value: 0.3 kappa score; similarity score > 0.3. b Heatmap colored arranged by p -values

Article Snippet: Sections were incubated overnight with RUNX1 antibody (Cusabio, China) at 4 °C.

Techniques: Functional Assay, Knockdown, Expressing, Control

Journal: Journal of Translational Medicine

Article Title: RUNX1 knockdown induced apoptosis and impaired EMT in high-grade serous ovarian cancer cells

doi: 10.1186/s12967-023-04762-8

Figure Lengend Snippet: The RUNX1 knockdown impacted apoptosis in the ovarian cancer cell via the FOXO1-Bcl2 axis. GSEA identified the enrichment plot of apoptosis-related genes in RUNX1 KD compared to control cells ( A ). Heat maps significantly compare the gene expression involved in apoptosis regulation in RUNX1 KD and control groups ( B ). Annexin V-PI staining analysis by flow cytometry showed that the knockdown of RUNX1 enhanced the apoptosis level of ovarian cancer cells ( C and D ). Western blot detected apoptosis-related molecules in RUNX1 knockdown cell lines ( E and F ). Western blot detected of apoptosis-related molecules after Ro5-3335 treatment ( G and H ). * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to control cells expressing a scramble shRNA control, paired t-test

Article Snippet: Sections were incubated overnight with RUNX1 antibody (Cusabio, China) at 4 °C.

Techniques: Knockdown, Control, Gene Expression, Staining, Flow Cytometry, Western Blot, Expressing, shRNA

Journal: Journal of Translational Medicine

Article Title: RUNX1 knockdown induced apoptosis and impaired EMT in high-grade serous ovarian cancer cells

doi: 10.1186/s12967-023-04762-8

Figure Lengend Snippet: RUNX1 knockdown affects EMT in ovarian cancer cells via the EGFR-AKT-STAT3 axis. Western blot detected the signaling molecules change pattern in the RUNX1 KD cells that involved in the EMT related signaling pathway ( A and B) . Western blot detected the signaling molecules change pattern after Ro5-3335 (a RUNX1 inhibitor) treatment that involved in the EMT related signaling pathway ( C and D ). Western blot detected of EMT-related molecules in RUNX1 knockdown cell lines ( E and F ). Western blot detected of EMT-related molecules after Ro5-3335 treatment ( G and H ). The expression of EMT-related molecules was analyzed by immunofluorescence in RUNX1 knockdown cell lines ( I and J ). * p < 0.05, ** p < 0.01 and *** p < 0.001 as compared with control cells expressing a scramble shRNA control, paired t test

Article Snippet: Sections were incubated overnight with RUNX1 antibody (Cusabio, China) at 4 °C.

Techniques: Knockdown, Western Blot, Expressing, Immunofluorescence, Control, shRNA

Journal: Journal of Translational Medicine

Article Title: RUNX1 knockdown induced apoptosis and impaired EMT in high-grade serous ovarian cancer cells

doi: 10.1186/s12967-023-04762-8

Figure Lengend Snippet: Correlation between six clinical drugs treated response and RUNX1 expression levels in ovarian cancer. RUNX1 expression levels in patients who responded to six clinical ovarian cancer agents versus patients who did not respond ( A ). Patients who responded to Avastin, paclitaxel, taxane, and platinum agents (cisplatin) had significantly lower levels of RUNX1 expression than those who did not respond. AUC and p-value of ROC of RUNX1 low expression level in treatment with clinical drugs ( B , C , D , and E ). There was no significant difference in RUNX1 expression between patients who responded to gemcitabine and docetaxel and those who did not reply ( F and G ). Effects on stably transfected sh-RUNX1 cell lines' proliferation after treatment with paclitaxel, taxane, and cisplatin ( H and I ). * p < 0.05, ** p < 0.01 and *** p < 0.001 as compared with control cells expressing a scramble shRNA control, paired t test

Article Snippet: Sections were incubated overnight with RUNX1 antibody (Cusabio, China) at 4 °C.

Techniques: Expressing, Stable Transfection, Transfection, Control, shRNA